Composite

Part:BBa_K1109000

Designed by: Berkhan GENÇ   Group: iGEM13_Baskent_Meds   (2013-09-23)


FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence with r

FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence with rbs in the 5' region of ORF. You can clone this part to downstream of a promoter, and fepA L8T mutant protein will be synthesized.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 256
    Illegal AgeI site found at 622
    Illegal AgeI site found at 1249
    Illegal AgeI site found at 1767
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None